Table 1. Functional interaction between the cap, Ω, TMV 3′-UTR and a poly(A) tail during translation.
| mRNA | Luciferase expression (light units/min/mg/protein) | ||
|---|---|---|---|
| Uncapped | GpppG | m7GpppG | |
| luc | 2940 | 35 400 | 63 000 |
| luc-TMV 3′ | 5210 | 727 000 | 1 870 000 |
| luc-A50 | 4480 | 407 000 | 1 330 000 |
| Ω-luc | 90 600 | 524 000 | 1 270 000 |
| Ω-luc-TMV 3′ | 201 000 | 32 600 000 | 65 300 000 |
| Ω-luc-A50 | 122 000 | 5 170 000 | 14 500 000 |
Capped or uncapped luc or Ω-luc mRNAs that were synthesized as poly(A)–, or terminating in a poly(A)50 tail, or the TMV 3′-UTR were delivered to carrot protoplasts. Expression from each mRNA construct was determined by measuring luciferase activity.