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. 2005 Aug;11(8):1281–1290. doi: 10.1261/rna.5040605

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FIGURE 3. — Expression of hTrm6p/hTrm61p restores wild-type levels of m¹A in tRNA. Total tRNA purified from wild-type yeast (Y200) containing pESC-URA (A), trm6-504 (Y190) containing pESC-URA (B), and trm6-504 (Y190) containing hTRM6/hTRM61(p230) (C) was digested to single nucleosides and analyzed by HPLC. The absorbance at 254 nm (AU₂₅₄) was measured and plotted against retention time in minutes, as previously described (Anderson et al. 1998). For clarity, only a portion of the chromatograph—the region where m¹A, 5-methylcytidine (m⁵C), and 2′-O-methylcytidine (C_m) elute from the column—is shown.