FIGURE 4.

Modification of specific tRNAs by hTrm6p/hTrm61p. Northern blot analysis of total RNA (5 μg) from a trm6Δ strain expressing htRNA₃ ^Lys and either TRM6 (TRM6), pESC-URA (trm6Δ), or hTRM6/hTRM61 (hTRM6/hTRM61). RNAs were separated on an acidic, denaturing 5% acrylamide/bis-acrylamide, 19:1, sequencing gel and transferred to a nylonmembrane for hybridization with radiolabeled probes specific for tRNA_i ^Met (A), htRNA₃ ^Lys (B), or tRNA^Leu(CAA) (C). Results were visualized by autoradiography.