TABLE 1.
HPLC analysis of tRNA
| Relative m1A | Relative m5C | |||
| Yeast strain/plasmid | m1A/Ψ | % Wild type | m5C/Ψ | % Wild type |
| Wild type/pESC-URA | 0.29 | 100 | 0.22 | 100 |
| trm6-504/pESC-URA | 0.02 | 6 | 0.21 | 98 |
| trm6-504/hTRM6/hTRM61 | 0.31 | 107 | 0.22 | 100 |
| trm61-2/TRM61 | 0.21 | 100 | 0.16 | 100 |
| trm61-2/pESC-URA | 0.02 | 10 | 0.16 | 100 |
| trm61-2/hTRM61 | 0.02 | 10 | 0.15 | 94 |
| trm61-2/hTRM6/hTRM61 | 0.15 | 71 | 0.18 | 113 |
The quantitative determination of m1A and 5-methylcytidine (m5C) levels was done by normalizing the percentage area of the m1A and m5C peaks to the percentage area of the pseudouridine (Ψ) peak in each sample. The amount of m1A and m5C found in the wild-type strain containing only pESC-URA and the trm61-2 strain complemented with TRM61 was set at 100%.