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. 2005 Aug;11(8):1291–1302. doi: 10.1261/rna.2060405

TABLE 2.

Plasmids used in this study

Plasmid Description Source
pAJ39 SKI2-2μ-LEU2 Brown et al. 2000
pAJ159 SKI2-3xmyc-2μ-LEU2 Brown et al. 2000
pAJ263 SKI3-LEU-CEN This study
pAJ267 GAL-SKI8-2μ-URA3 Brown et al. 2000
pAJ270 GAL-SKI3-2μ-TRP1 This study
pAJ689 GAL-GST-2μ-URA3 This study
pAJ703 GAL-SKI2-2μ-LEU2 This study
pAJ705 GAL-SKI2-3xmyc-2μ-LEU2 This study
pAJ706 GAL-GST-SKI8-2μ-URA3 This study
pAJ709 GAL-SKI3-3xHA-2μ-TRP1 This study
pAJ819 SKI2(DEVHAEVA)-3xmyc-2μ-LEU2 This study
pAJ820 GAL-SKI2(DEVHAEVA)-3xmyc-2μ-LEU2 This study
pAJ832 ski2ΔN-3xymc-2μ-LEU2 This study
pAJ833 GAL-ski3ΔC(aa 2-1206)-3xHA-2μ-TRP1 This study
pAJ840 ski3ΔC-LEU-CEN This study
pAJ951 SKI2 ORF in pGAD-C(1) This study
pAJ952 ski2(nt 1–840) in pGAD-C(1) This study
pAJ953 ski2(nt 841–1584) in pGAD-C(1) This study
pAJ954 ski2(nt 1585–2589) in pGAD-C(1) This study
pAJ955 ski2(nt 2590–3868) in pGAD-C(1) This study
pAJ956 SKI3 ORF in pGBD-C(1) This study
pAJ957 ski3(nt 1–1047) in PGBD-C(1) This study
pAJ958 ski3(nt 1048–1230) in pGBD-C(1) This study
pAJ959 ski3(nt 1231–1923) in pGBD-C(1) This study
pAJ960 ski3(nt 1924–2895) in pGBD-C(1) This study
pAJ961 ski3(nt 2896–3618) in pGBD-C(1) This study
pAJ962 ski3(nt 3619–4299) in pGBD-C(1) This study
pAJ963 SKI7 ORF in pGAD-C(1) This study
pAJ964 SKI8 ORF in pGAD-C(1) This study
pAJ965 SKI8 ORF in pGBD-C(1) This study
pAJ966 SKI7 ORF in pGBD-C(1) This study
pAJ1201 GAL-FLAG-SKI7-2μ-LEU2 This study
pAJ1206 GAL-FLAG-SKI7-2μ-URA3 This study
pAJ1224 pNMD3-ski3(aa 1207–1432)-3xHA-2μ-URA3 This study
pAJ1225 pNMD3-ski3(aa 966–1432)-3xHA-2μ-URA3 This study
pAJ1226 pNMD3-ski3(aa 642–1432)-3xHA-2μ-URA3 This study
pAJ1227 pNMD3-ski3(aa 411–1432)-3xHA-2μ-URA3 This study
pAJ1228 pNMD3-ski3(aa 350–1432)-3xHA-2μ-URA3 This study
pAJ1229 pNMD3-ski3(aa 2–1432)-3xHA-2μ-URA3 This study
pAJ1231 GAL-3xHA-2μ-TRP1 This study
pAJ1233 GAL-ski3(aa 2–349)-3xHA-2μ-TRP1 This study
pAJ1234 GAL-ski3(aa 2–410)-3xHA-2μ-TRP1 This study
pAJ1235 GAL-ski3(aa 2–641)-3xHA-2μ-TRP1 This study
pAJ1238 GAL-ski3(aa 1207–1432)-3xHA-2μ-TRP1 This study
pAJ1241 GAL-ski2(aa 1–279)-13xmyc-2μ-LEU2 This study
pRP469 GAL-PGK1-pG-2μ–URA3 Muhlrad and Parker 1994