FIGURE 3.

Glycerol gradient analyses of U5 snRNAs. Glycerol gradient sedimentation of Drosophila nuclear extract was performed on nuclear extract from Kc cells using 10%–30% linear glycerol gradients as previously described (Makarova et al. 2002). Fractions were analyzed by RNA blot hybridization using probes complementary to U1 snRNA (A), the conserved 5′ region of all U5 snRNAs (B), and U5 63BC (C). The Y-axis is the percent signal observed in each fraction over the sum of signal detected in all fractions.