FIGURE 5.
ChIP of active genes by ribosomal proteins is independent of gene translatability. (A) Radiolabeled PCR products from gal-inducible PGK1/LacZ fusion gene ± 5′-UTR hairpin and chromosomal GAL10 (G10) from cells co-expressing Rpl34bp-HAT and grown either in raffinose or galactose. ChIP was performed with α-RNA pol II (8WG16) and/or α-HA (12CA5), as indicated. (B) PCR products of snRNAs U1 and U2 (pol II transcripts), snRNA U6, and tRNALysCUU (pol III transcripts) from ChIP performed using α-HA (12CA5) on chromatin from HAT-tagged ribosomal proteins, including ChIP of chromatin from RPL34B-HAT and RPS7B-HAT cells treated or not with RNases A and T1 prior to IP.