FIGURE 3.
Progress of the compartmentalized in vitro selection. (A) Description of selection stringency and progress, by round. (B) Ligation activity of the pool. Activity was determined using a coupled transcription–ligation assay. A 5′ P32-labeled RNA substrate (SOH) was used in solution, without emulsification. Ligation products and substrates were at the correct molecular weight, as determined by their mobility relative to standards. The bands located between the “product” and the “substrate” were artifacts of T7 RNA polymerase acting on RNA substrates; these bands were also observed in the absence of a dsDNA template.