Figure 4.

Effects of the base paired with 5-foU on the cleavage of oligonucleotides by hNTH1. (A) The duplex oligonucleotide substrate, 5-foU/N (lanes 1–4) or T/N (lanes 5–8), was incubated with hNTH1 (8 ng) at 37°C for 3 min. N represents G (lanes 1 and 5), A (lanes 2 and 6), T (lanes 3 and 7) or C (lanes 4 and 8). The products were separated by denaturing 20% PAGE in gels containing 7 M urea. (B) The intensity of the upper band was analyzed using NIH Image software. Error bars show standard deviations.