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. 2005 Nov;11(11):1648–1654. doi: 10.1261/rna.2172105

FIGURE 3.

FIGURE 3.

K. lactis γ-toxin cleaves tRNAGlumcm5s2UUC, tRNALysmcm5s2UUU, and tRNAGlnmcm5s2UUG between position 34 and 35. (A) γ-toxin-GST– treated (+) or mock-treated (−) wild-type total tRNA was reverse transcribed by using 32P-labeled oligonucleotides complementary to the 3′-end of tRNAGlumcm5s2UUC, tRNALysmcm5s2UUU, or tRNAGlnmcm5s2UUG. The sequence ladder was derived from a gene coding for tRNAGlumcm5s2UUC. Arrowheads indicate reverse transcripts induced by γ-toxin treatment. (B) The purified 5′-half of tRNAGlumcm5s2UUC, tRNALysmcm5s2UUU, or tRNAGlnmcm5s2UUG was treated with the indicated enzyme(s) before ligation and RT-PCR amplification. (C) The sequence shared between tRNAGlumcm5s2UUC, tRNALysmcm5s2UUU, and tRNAGlnmcm5s2UUG in the anticodon region, and the site of γ-toxin cleavage.