TABLE 1.

Oligoribonucleotides used in this study

RNA	Sequence	Length
SL-1	GGCAU·CUUCGGAUGCAGGGGAGGCAGCUCCCGAUGGAAGUGACGAGCCAGCGUUCUCAACAGUAUUGACUGAACCUAAAAGCCAAUCGCAGGCUCAGC	98
SL-2	AGAACAAUACGUUAUAGUGACCAGGAAAAGACAAAUCUGCCCUCAGAGCUUGAGAACAU·AAAUA	64
SL-3	GGCAU·GCCAGCGUUCUCAACAGUAUUGACUGAACCUAAAAGCCAAUCGCAGGCUCAGC	58
SL-4	AGAACAAUACGUUAUAGUGACCAGGAAAAGACAAAUCUGCCCUCAGAGCUUGAGAAGAUCUUCGGAUCCAGGGGAGGCAGCUCCCGAUGGAAGUGACAU·AAAUA	104
SL-5	GGCAU·ACCAGCGUUCUCAACAGUAUUCACUGAACCUUAAAGCCAAUCGCAGGCUCAGC	58
SL-6	AGAAGAAUACGUUAUAGUGACCAGGAAAAGACAAAUCUGCCCUUAGAGCUUGAGAAGAUCUUCGGAUCCACGGGAGGCAGCUCGCGAUGGAAGUGACAU·AAAUA	104
S-163	d (CTTGACGTCAGCCTGGACTAATACGACTCA) UAUA	35

Underlined region is sequence complementary to the IGS region of the Azoarcus group I ribozyme; dot indicates expected cleavage and transesterification site. All SL oligos are written 5′–head · tail–3′ (e.g., A·B). Ligase ribozymes produced upon recombination are as follows: SL-1 × SL-2 = E100-3nRC; SL-3 × SL-4 = E100-3RC; SL-5 × SL-6 = B16-19RC. The chimeric DNA/RNA oligonucleotide S-163 is the substrate for ligase ribozymes.