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Kinetic characterization of class I ligase ribozymes in 10 mM MgCl2, 5 mM CaCl2, and 30 mM EPPS (pH 7.6) at 22°C
| Ribozyme | Origin | kcat (min−1) |
| E100–3 | Evolveda | 4.4 |
| B16–19 | Evolvedb | 3.6 |
| E100–3RC | Designed for recombination | 0.034 |
| B16–19RC | Designed for recombination | 0.033 |
aFrom Wright and Joyce (1997).
bFrom Schmitt and Lehman (1999).
