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. 2005 Dec;11(12):1835–1847. doi: 10.1261/rna.2179305

FIGURE 4.

FIGURE 4.

Degradation of the Drosophila DCP by a 5′–3′ exonuclease. (A) The sequence of the genuine Drosophila H3 pre-mRNA encompassing the stem–loop and the HDE. The arrow indicates the cleavage site. The upstream and the downstream cleavage products are indicated. (B) Processing of the uniformly labeled mH2a/RI (lane 3) or dH3* pre-mRNA (lanes 4,6,7) in a mouse or a Drosophila nuclear extract, respectively, showing generation of the upstream cleavage product and the release of mononucleotides from degradation of the DCP. (C) Degradation of the dH3-specific DCP incubated during the indicated time in a Drosophila nuclear extract (lanes 2–5) under control conditions (lanes 2,3) or in the presence of the αM or αDa oligonucleotides (lanes 4,5). The dH3 DCP incubated for 90 min in a mouse nuclear extract is shown in lane 6.