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. 2005 Dec;11(12):1898–1908. doi: 10.1261/rna.2192805

FIGURE 2.

FIGURE 2.

DMS modification of 40S subunits and 80S ribosomes. Sequencing lanes are shown to the left. Nucleotides with enhanced reactivity in 80S ribosomes are marked with a square; nucleotides protected in 80S ribosomes are marked with an arrow. Note that bands in the sample lanes appear one nucleotide below the corresponding sequencing band. Two 40S subunit reactions, two 80S ribosome reactions, and nonmodified (N) 18S rRNA are shown. Panels AD are taken from representative gels.