TABLE 1.
U65hp | Nop10p | |
Distance restraints | ||
Total NOE restraints | 572 | 743 |
Intraresidue | 186 | 354 |
Sequential (i + 1) | 288 | 191 |
Medium (i + 2 to i + 4) | 26 | 128 |
Long range (>i + 4) | 72 | 70 |
Hydrogen bond restraints | 64 | 10 |
RDC restraints | 11 | |
Dihedral angle restraints | 209 | |
Structure statistics | ||
No. of NOE violation >0.2 Å | 0.1 ± 0.0 | 4.6 ± 1.1 |
No. of NOE violation >0.5 Å | 0 | 0 |
Maximum violation, Å | 0.20 | 0.36 |
Mean deviation of NOE violation, Å | 0.022 ± 0.007 | 0.032 ± 0.010 |
Rms deviation from ideal covalent geometry | ||
Bond lengths, Å | 0.0044 ± 0.0012 | 0.0026 ± 0.0008 |
Angles, ° | 1.03 ± 0.27 | 0.47 ± 0.15 |
Impropers, ° | 0.39 ± 0.10 | 0.30 ± 0.10 |
Rms deviation (Å) from the mean structure | ||
Backbone | Heavy atoms | |
Nop10p (10 lowest energy structures) | ||
Residues 4–44 | 1.97 | 2.95 |
Residues 4–16 and 37–42 | 0.90 | 1.74 |
U65hp (15 lowest energy structures) | ||
Residues 1–34 | 2.38 ± 1.14 | 2.14 ± 1.03 |
Residues 9–26 | 1.23 ± 0.69 | 1.08 ± 0.59 |
Residues 1–6, 29–34 | 0.79 ± 0.22 | 0.78 ± 0.20 |