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Mismatch scanning assay. Generalized scheme for assaying unknown polymorphisms using a Endo V/ligation-based approach. In this example products are displayed using gel electrophoresis. In lane 2, dashed bands represent products due to spurious nicking which are sealed in the presence of thermostable ligase, lane 3. Abbreviations: Fam [5-(&6)-carboxyfluorescein]; Tet (tetrachloro-6-carboxyfluorescein).
