TABLE 1.

Primers used to introduce deletions into the g11 cDNA of SP65g11cn86^a

Deleted region	Forward primer	Reverse primer
2–50	ccggatatgcatttccctcaatttcttctagt	cggggcggccgccctatagtgagtcgtatta
2–100	ccggatatgcattcaactctttctggaaaatc	cggggcggccgccctatagtgagtcgtatta
50–150	ccggatatgcatgatgcagaagcattcaataa	cggggcggccgccgtcaatgctgagagacatc
100–200	ccggatatgcattggaccatctgattctgctt	cggggcggccgcacgttgtagaagatgattca
150–250	ccggatatgcatgatcgaatgcagttaagaca	cggggcggccgctggtgaaatgtactgttcac
200–300	ccggatatgcattcaacgcaatcacgaccttc	cggggcggccgcatatcctctggagacttcga
250–350	ccggatatgcatctccttaactaaaggtgtta	cggggcggccgctaatcgaaaagctggtgagt
300–400	ccggatatgcatcaatctcaactgattataag	cggggcggccgctgaatccatagacacgccag
350–450	ccggatatgcataaacactacccaagaattga	cggggcggccgcaaatccacttgatcgcaccc
400–500	ccggatatgcatagatgattcagacagtgatg	cggggcggccgcatatacatgaatcaagatta
450–550	ccggatatgcatagaagtatttcgcacttaga	cggggcggccgccctatttttatcctttttgg
500–600	ccggatatgcatatcgaagatttgtaatgtca	cggggcggccgcaaaacgtaatcttcggaatc
550–650	ccggatatgcatcccgttttgtgaccgcgg	cggggcggccgctcttatatttacaattctta
600–650	ccggatatgcatcccgttttgtgaccgcgg	cggggcggccgccaattgcattgcgacttgct
650–664	ccggatatgcatgaattccgtgtattctatag	cggggcggccgcagtggggagctccctagtgt

^aForward and reverse primers were used in polymerase chain reactions to delete the indicated residues in the g11 cDNA of the vector SP65g11cn86. Forward and reverse primers contained NsiI and NotI sites, respectively (underlined).