FIGURE 4.

Influence of base-pairing length on reporter activation. (A) Sequences of the different RNAs tested. Variants of X₅₀ and Y₅₀ were designed to interact over different lengths with a central sequence corresponding to the six interacting base pairs of X₁ and Y₁. The sequence shown encompasses the cloning sites and part of the stem enclosing the interacting loops. (B) Quantitative analysis of β-galactosidase activity of the different pairs. The activity is normalized relative to the activity of X₁–Y₁ (1.0). Error bars indicate standard deviation. X₅₀–Y₅₀ indicates YBZ-1 strain transformed with plasmids expressing RNAs X₅₀ and Y₅₀; numbers 6–50, YBZ-1 strain transformed with plasmids expressing RNAs X₅₀mod-6 to X₅₀mod and Y₅₀mod.