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. 2006 Feb;12(2):198–205. doi: 10.1261/rna.2281306

TABLE 1.

Plasmids and yeast strains

Name Details of plasmid/genotypes of yeast strains
pMod-Tn PRP8 transposon plasmid. Contains PRP8 downstream flanking region, KanMX4 cassette, PRP8 upstream flanking region, SV40 NLS.
pBS1479 PCR template for TAP-tagging (Rigaut et al. 1999).
SC261Δ8B1 MATa, ura3-52, leu2, trp1, pep4-3, prb1-1132, prc1-407, prp8::Blasticidin [pRS316-PRP8]
BMA38a MATa, his3Δ200, leu2-3,112, ura3-1, trp1Δ1, ade2-1, can1-100
RG8T PRP8:TAP-TRP1, otherwise as BMA38a
RG8T/2173 PRP8(1-2173):Tn:PRP8(2174-2413):TAP-TRP1, otherwise as BMA38a
KL8T/770 PRP8(1-770):Tn:PRP8(771-2413):TAP-TRP1, otherwise as BMA38a
KL8TG/436 His3-PGAL1-HA3:PRP8(1-439):Tn:PRP8(437-2413): TAP-TRP1, otherwise as BMA38a