Skip to main content
. 2006 Feb;12(2):213–222. doi: 10.1261/rna.2820106

FIGURE 3.

FIGURE 3.

40LoVe coimmunoprecipitates three proteins from the Yolk Pellet Wash fraction. (A) Eluate from a large-scale immunoprecipitation was analyzed on a 10%–13% gradient SDS-PAGE gel. The position of 40LoVe in the gel is indicated. The location of three proteins specifically present in the 40LoVe IP is indicated by arrowheads. Sequencing of the Vg1RBP/Vera band yielded five peptides, all corresponding to Vg1RBP/Vera. The 35-kDa band yielded three peptides, all matching the Xenopus-hnRNP D/AUF1 protein (Accession AY640106). The 100-kDa band could not be identified. (B) The supernatant of the immunoprecipitation was analyzed by Western blotting for the presence of both Vg1RBP/Vera and 40LoVe (indicated to the right). (C) An aliquot of the immune-precipitates was analyzed to confirm the presence of 40LoVe and Vg1RBP/Vera in the precipitate. The weak 40-kDa signal in the IgG lane is due to a degradation product of control IgG present in this antibody preparation.