FIGURE 4.

40LoVe interacts with GST-hnRNP I. (A) Glutathione resin bound to either GST-λ or GST-hnRNP I was incubated in Xenopus ovary extract (Czaplinski et al. 2005) and bound proteins analyzed by Western blotting against 40LoVe or VgRBP71 as indicated. Beads were washed with or without RNAse A as indicated. A short and long exposure of the same 40LoVe blot is shown to reflect the quantitative differences in bound 40LoVe. (B) Purified recombinant GST-λ, GST-hnRNP I, and 6His-40LoVe were combined as indicated, and then total bound (B) and one half of unbound fractions (U) were analyzed by SDS-PAGE gel followed by Coomassie blue staining.