Comparison of D5-PL and D5-Ai5γ NMR structures. (A) Stereoview of the superposition over all heavy atoms of the 10 lowest energy structures of the bulge nucleotides (U9, A24, [excluding A25], G26) and surrounding base pairs (G8–C27 and G10–C23) within D5-PL. (B) Stereoview of the superposition over all heavy atoms of the 10 lowest energy structures of the bulge nucleotides (U9, A24, [excluding C25], and G26) and surrounding base pairs (A8–U27 and G10–C23) within D5-Ai5γ. (C) Stereoview of the superposition of representative (structure closest to the mean structure) D5-PL and D5-Ai5γ bulge structures (nucleotides 8–10, 23, 24, 26, 27).