TABLE 1.

Structural statistics for D5-PL

	With RDC	Without RDC
A. Restraints for structure calculations
No. of structures
Accepted	10	30
Calculated	30	100
No. of intraresidue distance restraints	349	349
No. of interresidue distance restraints	200	200
No. of dihedral restraints	304	304
No. of hydrogen-bond restraints	60	60
No. of dipolar coupling restraints	37	0
B. Average RMS deviation of experimental data
Average RMS deviation of distance (Å)	0.019	0.018
Average RMS deviation for dihedral angle (L)	0.676	0.528
Average RMS deviation for RDC (Hz)	2.5	0
C. Average RMS deviation from idealized geometry
Bonds (Å)	0.017	0.018
Angles (L)	0.628	0.659
Improper(L)	0.344	0.330
D. Violations
NOE violations >0.2 A °	0	0
Dihedral violations >5L	0	0
E. Heavy atoms average RMS deviation from the mean structure and (RMS pairwise difference between structures) (Å)
Overall	0.65 ± 0.13 (0.97 ± 0.20)	1.34 ± 0.38 (1.99 ± 0.52)
Lower helix (residues 1–8, 27–34)	0.43 ± 0.07 (0.64 ± 0.14)	0.72 ± 0.13 (1.07 ± 0.27)
Upper helix (residues 10–14, 19–23)	0.33 ± 0.07 (0.49 ± 0.11)	0.63 ± 0.16 (0.93 ± 0.22)
Tetraloop (residues 15–18)	0.34 ± 0.07 (0.51 ± 0.18)	0.47 ± 0.08 (0.70 ± 0.16)
Bulge (residues 9, 24, 25, 26)	1.35 ± 0.16 (2.00 ± 0.34)	1.42 ± 0.42 (2.12 ± 0.66)
Bulge (residues 9, 24, 26)	0.86 ± 0.07 (1.28 ± 0.34)	1.42 ± 0.42 (2.12 ± 0.66)