TABLE 3.

Equilibrium dissociation constants (K_d) and free-energy differences (ΔΔG) for binding of wild-type and mutant PSI-KH03 proteins to PSI29

Enzyme	Kd (nM)a	ΔΔG
Wild-type	22 ± 7	n/a
I145N	100 ± 30	−0.84
I234N	1300 ± 200	−2.3
I338N	500 ± 100	−1.7
I449N	160 ± 20	−1.1

^aAll K_d measurements are the average of at least three separate experiments, with errors reported as one standard deviation from the average.

^bNot applicable.