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. 2004 Mar;10(3):378–386. doi: 10.1261/rna.5169404

FIGURE 7.

FIGURE 7.

Analyses of protein and RNA derived from the crp–crr fusion genes. (A) Western blot analysis of CRP proteins. Lysates equivalent to OD600 = 0.005 unit prepared from TA341 (Δcrp ssrA+), TA501 (Δcrp ΔssrA), or TA481 (Δcrp ssrADD) cells harboring pST602 (lanes 1–3) and pJK107 (lanes 4–6) were analyzed by Western blotting using anti-CRP antibodies. (B) Northern blot analysis of crp mRNAs derived from the crp–crr fusion genes. Total RNA (1 μg) prepared from TA341 (Δcrp ssrA+) and TA501 (Δcrp ΔssrA) cells harboring pST602 (lanes 1,2) and pJK107 (lanes 3,4) were resolved by electrophoresis on a 1.5% agarose-formaldehyde gel. Northern blot analysis was performed using the DIG-labeled crp probe. RNA bands corresponding to the full-length crp–crr and truncated crp mRNAs are indicated by arrowheads. (Lane M) RNA size markers. (C) Northern blot analysis of crr mRNAs derived from the crp–crr fusion genes. Total RNA (1 μg) prepared from TA341 (Δcrp ssrA+) and TA501 (Δcrp ΔssrA) cells harboring pST602 (lanes 1,2) and pJK107 (lanes 3,4) were resolved by electrophoresis on a 1.5% agarose-formaldehyde gel. Northern blot analysis was performed using the DIG-labeled crr probe. RNA bands corresponding to the full-length crp–crr and truncated crr mRNAs are shown by arrowheads. (Lane M) RNA size markers.