TABLE 3.
Transfer RNA-dependent stimulation of editing by E. coli ValRS
| Analog substitution | Rate of ATP hydrolysis (pmole/min)a | Relative rate of ATP hydrolysis | Steady-state level of misacylation with threonine (pmole/A260) |
| Adenosine | 845 | (1.0) | 0 |
| 3-Deazaadenosine | 823 | 0.97 | 6 |
| 7-Deazaadenosine | 654 | 0.77 | 12 |
| Purine Riboside | 675 | 0.80 | 14 |
| Inosine | 17 | 0.02 | 153 |
| 2-Aminopurine | 20 | 0.02 | 429 |
| 2,6-Diaminopurine | 208 | 0.25 | 9 |
| Xanthosine | 10.2 | 0.01 | 34 |
| Isoguanosine | 169 | 0.20 | 381 |
| 7-Deazaguanosine | −48 | −0.06 | 12 |
| Uridine | −25 | −0.03 | 1628 |
| Cytidine | −88 | −0.10 | 1312 |
| Guanosine | −29 | −0.03 | 36 |
| No tRNA | −51 | −0.06 | — |
aNegative values are the result of small variations in the background rate of ATP hydrolysis.