Figure 4.

(A) ELISA reactivity of phage affinity selected from the original λDHB (non ORF) and ORF-enriched (ORF) libraries with mAb GAP-43. Pools of phage obtained after each round of selection–amplification (Ist, IInd) were tested, and compared with the reactivity of the unselected libraries (Uns.). Average values (A_405nm) from two independent experiments were determined. (B) Frequency of proteins (black bars) and mimotopes (gray bars) among mAb GAP-43-positive clones selected from the λDHB library (non ORF) and ORF-enriched (ORF) libraries. Proteins refer to cDNA inserts with the correct reading frame and encoding domain of naturally occurring proteins. Mimotopes refer to cDNA inserts in a non-correct orientation, or with no homology with known proteins.