Figure 5.

Identification of an N-terminal oligomerization domain in EcTERT. (A) Untagged full-length EcTERT was co-expressed with T7-tagged N186 and N654 in the presence of [³⁵S]methionine. Immuno precipitates (lanes 3 and 4) and unbound fractions (lanes 1 and 2) are shown. (B) T7-tagged C-terminal truncation mutants were co-expressed with full-length EcTERT in the presence of ³⁵S and then subjected to immunoprecipitation (lanes 1–6). In lane 7, T7-tagged, unlabeled N186–354 was incubated with untagged, ³⁵S-labeled full-length EcTERT, subjected to immunoprecipitation and run on a separate gel.