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Quenching of AEDANS fluorescence emission by acrylamide. Quenching of AEDANS probe (cross), quenching of AEDANS from labelled ocr(Cys) mutant proteins (filled circles) and quenching from labelled ocr(Cys) proteins in the presence of M.EcoKI (open circles). Ocr(Cys) data are coloured as follows: ocr(N4C), black; ocr(D25C), red; ocr(N43C), orange; ocr(D62C), green; ocr(S68C), cyan; ocr(W94C), blue.
