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. 2005 Sep 16;6(11):1045–1051. doi: 10.1038/sj.embor.7400534

Figure 2.

Figure 2

Filamin interacts with the intracellular domain of epithin. (A) Comparison of Y2 with the carboxyl termini of mouse filamin-B, human filamin-B and human filamin-A. Grey-boxed residues represent sequence identities, and residues inside the black line represent sequence homology. (B) 6 × histidine (His)/thioredoxin-tagged Y2 protein tested for interaction with glutathione S-transferase (GST) or GST–epithin intracellular domain (EICD) fusion protein. The arrows indicate the 6 × His/thioredoxin-tagged Y2 probed with anti-His tag monoclonal antibody. GST and GST–EICD proteins used for pull-down of Y2 were visualized nonspecifically with anti-His tag antibody owing to the large amounts of protein. (C) After transfection of epithin complementary DNA into COS7 cells, cell lysates were immunoprecipitated (IP) with normal rabbit serum (Ctrl) or anti-N antiserum, and blotted (IB) with anti-filamin antibody or anti-N antiserum, as indicated.