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. 2002 Sep 15;30(18):4075–4087. doi: 10.1093/nar/gkf529

Figure 1.

Figure 1

A large DNA–protein complex is resolved by an agarose gel EMSA and its formation of the complex is DNA-PK dependent. EMSAs were performed using nuclear extracts (0.5 µg) from human glioblastoma cell lines M059K or M059J in the presence of closed circular pUC18 (1 mg) and 32P-labeled 144 bp probe (1 ng) derived from pUC18. Reactions were separated in a 5% PAGE gel (lanes 1–3) or 1.5% agarose gel (lanes 4–8), dried, and subjected to autoradiography. A parallel agarose gel was stained with 1 mg/ml ethidium bromide and photographed (lanes 9–13). Purified DNA-PKcs/Ku (30 ng) was added to reactions either alone (lanes 8 and 13) or containing M059J nuclear extract (lanes 7 and 12). Black arrows indicate the positions of Ku-bound probe; the white arrow the position of the large DNA-PK-dependent end-binding complex.