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. 2002 Sep 15;30(18):4075–4087. doi: 10.1093/nar/gkf529

Figure 3.

Figure 3

Complex formation in Chinese hamster DSB repair mutants. Agarose gel EMSAs were performed using nuclear extracts (5 µg) from Chinese hamster cell lines CHO, AA8, V3 (DNA-PKcs–), V3-YAC147 (DNA-PKcs+) and xrs5 (Ku86–) as described in Figure 1. A large complex was observed (large EB complex, upper black arrow) in extracts from parental lines CHO and AA8, but not in mutant lines lacking Ku86 (xrs5), or the catalytic subunit of DNA-PK (V3). In contrast, the V3-YAC147 cell line that contains a YAC expressing human DNA-PKcs produced an intense band at the position of the large complex. Lower black arrows identify the Ku bands.