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. 2002 Sep 15;30(18):4022–4031. doi: 10.1093/nar/gkf531

Figure 4.

Figure 4

Analysis of DNA contents and the percentage of cells showing the ‘cut’ phenotype following release from HU arrest. (A) After S-phase arrest (10 mM HU at 37°C for 2 h) cells were fixed for FACS analysis at 20 min intervals. (B) rqh1-d, top3rqh1 double and top3-ts mutant cells were treated with 10 mM HU as above and then incubated at 30°C for 4 h in fresh YES media. Cells were sampled every 20 min for DAPI staining. The proportions of cells showing the ‘cut’ phenotype were plotted against the incubation times after HU removal. (C) The minichromosome loss rates per generation for rqh1 and top3-ts mutants.