Table 1. Percent seedlings displaying silencing of the nptII gene in lines transformed with the constructs indicated.
Construct | Line | Silencing (%) |
---|---|---|
pKOH 110 ex2–4a | 4 | 3.8–29.6 |
5 | 5.7b | |
pPCV002 Pl:CpGa | 19 | 3.5–7.0 |
20 | 8.5–10 | |
pKOH110 35SGUS | K11c | 0.7–4.2 |
K13 | 100d | |
K14 | 4.2–50 | |
K15 | 2.7–15.2 | |
K16 | 6.8d | |
pPCV002 35SGUS | P4c | 1.7–100 |
P6c | 28.1–50 | |
P9 | 12.9–17.8d | |
P10 | 3.0–10.7 |
The silencing level varies between siblings and generations, and the lowest and highest observed percentage silencing is therefore given for each line. For each line, 60–150 seeds from up to 23 siblings and up to five generations were tested.
aLines reported by Meza et al. (26).
bSilencing was only observed in the T5 generation of ex2–4 line 5.
cLines reported by Meza et al. (24).
dProgeny of one sibling only of lines K13 and K16, and two siblings of line P9, displayed silencing.