Figure 5.
Repression by TTK of GAGA-dependent activation requires the POZ/BTB domain of GAGA. S2 cells were co-transfected with a reporter plasmid carrying a luciferase gene fused to the 5×GAL4-hsp70 promoter (see Materials and Methods) and the amounts indicated of plasmids overexpressing GAL4Q (A) or POZGAGAGAL4Q (C), or with the same reporter plasmid and either 2 µg of the plasmid overexpressing GAL4Q (B) or 8 µg of the plasmid overexpressing POZGAGAGAL4Q (D) and the amounts indicated of the plasmid overexpressing TTK. The data correspond to the average of six independent experiments.