Table 2. Meiotic divisions in strains containing motif IV mutations.

	Time after shift to sporulation medium (h)
	2	5	8	11	13.5	24	30	48
Gene on plasmida
None	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
Wild-type	0.0	0.0	23.0	38.3	38.4	54.6	54.6	54.8
D348A	0.0	0.0	0.4	2.8	2.8	6.0	6.4	7.5
W351A	0.0	0.0	0.3	0.6	0.8	1.0	0.3	0.3

Transformants of the ime4-deficient MC309 strain were grown pre-sporulation medium and shifted to sporulation medium. Each culture was sampled at the hourly intervals shown for DAPI staining and epifluorescence microscopy. The percentage of cells containing two or more nuclear masses was determined by counting at least 600 cells for each time point shown. For the D348A and W351A strains, at least 800 cells were counted.

^aWild-type and mutant genes were carried on the CEN-URA3 vector, pRS316.