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. 2002 Oct 15;30(20):4470–4480. doi: 10.1093/nar/gkf575

Figure 3.

Figure 3

Effect of deleting residues 56C–52C from the 3′ end of the alignment domain on telomerase activity by TRAP assay. Lane 1, 1× CHAPS buffer; lane 2, TSR8 template amplification control; lane 3, wild-type telomerase from HeLa cell extracts; lane 4, RRL control (–hTR, –hTERT mRNA); lane 5, telomerase activity reconstituted from wild-type hTR; lanes 6–10, recombinant mutants Δ1, Δ2, Δ3, Δ4 and Δ5, respectively; lane 11, RRL control (+hTR, –hTERT mRNA); lane 12, RRL (–hTR, +hTERT mRNA). The internal PCR control band (I.C.) for the assay is indicated.