Table 1. Phosphorothioate immunomers and related CpG DNAs showing the number and position of linker.
| CpG DNA number | Sequence | Linker (X)a | Lengthb | Molecular Weight | |
|---|---|---|---|---|---|
| Calculated | Foundc | ||||
| 10 | 5′-CTATCTGACGTTCTCTGT-3′ | None | 18mer | 5704 | 5705 |
| 11 | (5′-CTATCTGACGTTCTCTGT)2-X-C-5′ | Glyceryl linker (2) | 18mer | 11945 | 11943 |
| 12 | (5′-CTATCTGACGTTCTCTGT)2-X-C-5′ | Long linker (3) | 18mer | 12158 | ND |
| 13 | (5′-CTATCTGACGTTCTCTGT)3-X | Long linker (3) | 18mer | 17635 | ND |
| 14 | 5′-CTGACGTTCTCTGT-3′ | None | 14mer | 4429 | 4428 |
| 15 | 5′-CTGACGTTCTCTGT-X-TGTCTCTTGCAGTC-5′ | Glyceryl linker (2) | 14mer | 9106 | ND |
| 16 | (5′-CTGACGTTCTCTGT)2-X-C-5′ | Long linker (3) | 14mer | 9609 | ND |
| 17 | 5′-TCTGACGTTCTCTGT-3′ | None | 15mer | 4749 | 4746 |
| 18 | 5′-TCTGACGTTCT-3′ | None | 11mer | 3458 | 3457 |
| 19 | 5′-GACGTTCT-3′ | None | 8mer | 2513 | 2512 |
| 20 | 5′-CTGACGTTCTCTG-X-GTCTCTTGCAGTC-5′ | Glyceryl linker (2) | 13mer | 8465 | 8455 |
| 21 | 5′-CTGACGTTCTCT-X-TCTCTTGCAGTC-5′ | Glyceryl linker (2) | 12mer | 7775 | 7772 |
| 22 | 5′-TCTGACGTTCT-X-TCTTGCAGTCT-5′ | Glyceryl linker (2) | 11mer | 7164 | 7166 |
| 23 | 5′-CTGACGTTCT-X-TCTTGCAGTC-5′ | Glyceryl linker (2) | 10mer | 6524 | 6522 |
| 24 | 5′-TGACGTTCT-X-TCTTGCAGT-5′ | Glyceryl linker (2) | 9mer | 5914 | 5913 |
| 25 | 5′-GACGTTCT-X-(T-5′)TCTTGCAG-5′ | Glyceryl linker (2) | 8mer | 5594 | 5593 |
| 26 | 5′-TCTGAGCTTCT-X-TCTTCGAGTCT-5′ | Glyceryl linker (2) | 11mer | 7164 | 7166 |
| 27 | 5′-CTGACGTTCT-X-TCTTGCAGTC-5′ | Phosphorothioate (1) | 10mer | 6354 | 6354 |
| 28 | 5′-CTGACGTTCT-X-TCTTGCAGTC-5′ | C2-linker (4) | 10mer | 6494 | 6519 |
| 29 | 5′-CTGACGTTCT-X-TCTTGCAGTC-5′ | C3-linker (5) | 10mer | 6508 | 6503 |
| 30 | 5′-CTGACGTTCT-X-TCTTGCAGTC-5′ | C4-linker (6) | 10mer | 6522 | 6515 |
| 31 | 5′-CTGACGTTCT-X-TCTTGCAGTC-5′ | C12-linker (7) | 10mer | 6634 | 6629 |
| 32 | 5′-CTGACGTTCT-X-TCTTGCAGTC-5′ | Spacer18 (8) | 10mer | 6714 | 6710 |
| 33 | 5′-CTGACGTTCT-X-TCTTGCAGTC-5′ | Abasic linker (9) | 10mer | 6550 | 6547 |
| 34 | 5′-CTGACGTTCT-XX-TCTTGCAGTC-5′ | C3-linker (5) | 10mer | 6662 | 6660 |
| 35 | 5′-CTGACGTTCT-XXX-TCTTGCAGTC-5′ | C3-linker (5) | 10mer | 6816 | 6814 |
| 36 | 5′-CTGACGTTCT-XX-TCTTGCAGTC-5′ | Abasic linker (9) | 10mer | 6746 | 6743 |
| 37 | 5′-CTGACGTTCT-XXX-TCTTGCAGTC-5′ | Abasic linker (9) | 10mer | 6942 | 6939 |
| 38 | 3′-C-X-(CTATCTGACGTTCTCTGT-3′)2 | Glyceryl linker (2) | 18mer | 11945 | ND |
| 39 | 5′-CTGACGTTCTCTGTCTTGTCCTGACGTTCTCTGT-3′ | None | 34mer | 10852 | ND |
aSee Figure 1A for chemical structures of linkers 1–9.
blength of each segment in 3′–3′- or 5′–5′-linked CpG DNA excluding linker.
cND, not determined.