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. 2002 Oct 15;30(20):e110. doi: 10.1093/nar/gnf109

Table 1. Summary of deletion screens.

Screen no. Sequence screened Size scanned (kb) No. of initial positives No. of positives rescreened Single plate ID No. of clonal positives Allele isolated Deletion size (bp)
1 kqt-1, 5′ 2.4 4/96 0/4        
∼100 000 genomes slo-2, 5′ 1.7 3/96 1/3 Yes 2/∼1056 Nonea 1554a
  slo-2, pore 2.5 2/96 0/2        
  slo-2, 3′ 2.1 7/96 1/7 Yes 3/192 slo-2(nf100) 574
  shl-1, 5′ 2.8 0/96          
  shl-1, 3′ 2.4 0/96
  
        
      16 1/16 (2/16)b        
                 
  kqt-1, 3′ 3.2 1/12 0/1        
  kqt-2, 3′ 2.1 1/12 0/1        
  kqt-2, whole 3.1 0/12          
  kqt-3, whole 3.1 2/12
 1/2
 Yes 1/336 kqt-3(aw1) 596
      4 1/4        
                 
2 shl-1, 5′ 2.8 5/96 0/5        
∼100 000 genomes shl-1, 3′ 2.4 7/96
 0/7
        
      12 0/12        
                 
  kqt-1, 5′ 2.4 0/12          
  kqt-1, whole 7.2 1/12 0/1        
  kqt-2, 5′ 0.9 0/12          
  kqt-2, whole 3.1 1/12 1/1 Yes 2/292 kqt-2(aw2) 1232
  kqt-3, whole 3.1 1/12 0/1        
  shw-3 3.2 1/12 0/1        
  slo-2, pore 2.5 0/12          
  slo-2, 5′ and 3′ multi 3.8 2/12 1/2 Yes 2/192 slo-2(nf101) 372
  shl-1, 5′ 2.8 0/12          
  shl-1, 3′ 2.4 0/12
  
        
      6 2/6        
                 
3 kqt-1, 5′ 2.4 1/12 1/1 Yes 1/197 kqt-1(aw3) 620
∼200 000 kqt-1, 3′ 3.2 1/12 0/1        
genomes kqt-1, whole 7.2 1/12 0/1        
  kqt-2, 5′ 0.9 2/12 0/2        
  kqt-2, whole 3.1 2/12 0/2        
  kqt-3, whole 3.1 2/12 1/2 Yes 1/58 kqt-3(aw4) 1674
  shw-3 3.2 1/12 0/1        
  slo-2, pore 2.5 0/12          
  shl-1, 5′ 2.8 3/12 0/3        
  shl-1, 3′ 2.4 1/12 0/1        
  shl-1, whole 4.7 0/12          
  shl-1, 5′ (TliI) 1.5 0/12          
  shk-1, 5′ 1.9 1/12 0/1        
  shk-1, 3′ 2.2 3/12
 1/3
 Yes 0/384c    
      18 3/18        

aStrain failed to show Mendelian segregation.

bIncluding strain that failed to show Mendelian segregation.

cFailed to isolate clonal strain.