Figure 4.

Overexpression of dbpA and dbpB/YB-1 CSD proteins represses expression from the VEGF HR region. VEGF HR region reporter constructs (pTKV5Luc and pTKV7Luc) were co-transfected with dbpA (pSGdbpA) or dbpB/YB-1 (pSGdbpB) overexpression vectors or the pSG5 overexpression backbone vector, into Balb/c 3T3 fibroblasts. Cells were grown under normoxic (unstimulated) or hypoxic (activated) conditions and assayed for luciferase activity. Percent repression represents the degree to which luciferase activity of each HR region reporter construct is reduced when co-transfected with the pSGdbpA (dbpA) or pSGdbpB (dbpB/YB-1) overexpression vector, relative to luciferase activity from reporter constructs co-transfected with the backbone overexpression vector (pSG5) alone. Data is pooled from four experiments. Luciferase activity from the backbone reporter vector was not affected by dbpA or dbpB overexpresssion.