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(A) The scheme of the DNA hemiknot structure formation. The novel structure is formed during annealing of two complementary strands with CA/TG repeats. The formation of a slipped CA/TG duplex is a critical step for the conversion of the interlocked looped intermediate to the stable hemiknot structure. (B and C) Schemes illustrating how the parameters for the 441 (B) and 573 bp (C) designs were measured.
