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Effect of PPG on G quenching of fluorescein. (A) Structure and relative fluorescence of FAM-nucleotide monomers. The indicated compounds (0.02 µM in 1× PBS, pH 7.2) were analyzed (excitation = 494 nm, emission = 522 nm. (B) Effect of PPG substitution in the 5′-nuclease assay on fluorescent signal. g = PPG.
