Table 1. PPG increases substitution on T_m of ODNs.

	5′-Probe Sequence-3′	g content	aTm°C	Tm°C	bΔTm/base
			X = PPG	X = G
1	CCAACACTCXTXAA	2/14	54.9	52.9	1.0
2	CCACCCXCCTCAX	2/13	60.7	59.7	0.5
3	CTXTAAXTAXATATAAC	3/17	51.8	49.5	0.8
4	XATTACCTXXATTT	3/14	50.6	48.8	0.6
5	XTAAXTAXACACAXC	4/15	59.5	53.5	1.5
6	XTAAXTAXXCATAAC	4/15	55.7	53.2	0.6
7	CXXCTACATXCTXX	5/14	61.1	58.8	0.5
8	XTAAXTAXACXAXC	5/15	62.9	56.7	1.2
9	XTAAXTAXXCXCAXC	6/15	65.5	58.2	1.2
10	CAXXXAXCTTTXXA	6/14	59.9	56.4	0.6

^aMelting temperature (T_m) of DNA duplexes formed with ODNs containing G or PPG at the X positions. The accuracy of the T_m determination is ±0.2°C according to the instrument manufacturer (Cary 400 Bio UV/Vis Spectrophotometer, Varian, Palo Alto, CA).

^bΔT_m/base is the average increase in T_m per base when G is substituted with PPG.