Table 2. PPG substitution improves mismatch discrimination (ΔT_m) in self-complementary duplexes.

^aBold nucleotides (N) opposite X (G or PPG) in the self-complementary. ODNs give the indicated mismatch.

^bODN concentration was 5 × 10^–6 M in 1 M NaCl, 10 mM Na cacodylate, pH 7.0.

^cΔT_m = T_m(match) – T_m(mismatch), a measure of probe specificity.