Table 3. Effect of PPG substitution on electrophoretic mobility of MGB-ODN conjugates.

	aSequence	GC content	bRm	Rm
			X = G	X = PPG
A	d(ACCTGTATTCCTTGCC)	8/16	1.00
B	d(XTACAXCAAATXXAA)	6/15		1.00
11	d(CAAATXXXXXXXXX)	10/14	0.58	0.96
12	d(ACAAATXXXXXXXX)	9/14	0.42	0.97
13	d(AACAAATXXXXXXX)	8/14	0.37	0.95
14	d(CAACAAATXXXXXX)	8/14	0.35	0.95
15	d(ACAACAAATXXXXX)	7/14	0.32	0.98
16	d(CACAACAAATXXXX)	7/14	0.29	1.03
17	d(CACAACAAATXXX)	6/13	0.96	0.98
18	d(AGCACAACAAATXX)	6/14	0.96	0.96

^aMGB-ODNs contained 5′-fluorescein, 3′-Q-MGB as shown in Figure 3. In sequence B, X = PPG. Sequences 11–18 contained either G or PPG at the indicated X positions.

^bMobility relative to control (R_m) of G- and PPG-containing ODNs were measured by non-denaturing PAGE. R_m values were measured separately for G- and PPG-containing ODNs with respect to control ODNs A and B, respectively.