Table 4. Genes identified in a two-hybrid screen using pLEXAPRP19/PSO4 as bait.
| Gene |
Gene informationc |
Extent of fusiond |
|---|---|---|
| RAD2 (5)a | DNA repair; DNA endonuclease; repairosome | C-terminal second half of the protein |
| SWM1 (4) | Nuclear; required for the last phase of sporulation | Contains the whole ORF |
| YNL018c (3)b | Unknown function | Starts at amino acid 10 |
| HSL1 (2) | Cell cycle control, C156 and C111 | C-terminal two-thirds of the protein |
| YLR132c (2) | Unknown function, essential, induced during meiosis | Contains the whole ORF; starts at amino acid 2 |
| DBR1/PRP26 (1) | Lariat RNA splicing debranching enzyme | C-terminal third of the protein |
| MDS3 (1) | Meiosis; regulator of early meiotic genes, C16, C46 and C106 | C-terminal quarter of the protein |
| NUD1 (1) | Cell cycle regulatory protein, essential, C106 | C-terminal two-thirds of the protein |
| PLO1 (1) | Unknown function, weak similarity to Nip80p, C152 (the same as RAD16 and RAD52) and C185 | C-terminal half of the protein |
| RLF2 (1) | Chromatin assembly complex, subunit p90 | C-terminal two-thirds of the protein |
| YAL028w (1) | Interacts with EST1, a telomere elongation protein | C-terminal; starts at amino acid 494 |
| YDR124w (1) | Unknown function | C-terminal third of the protein |
| YML083c (1) | Unknown function | C-terminal half of the protein |
aFrequency of independent isolation is shown in parentheses.
bC indicates association of the respective isolate with the protein complex from the Yeast protein complex database (http://yeast.cellzome.com/).
cAccording to Mewes et al. (28) and Costanzo et al. (44), SGD (http://genome-www.stanford.edu/Saccharomyces/), and the Yeast protein complex database (http://yeast.cellzome.com/).
dSegment of each ORF fused to the transcriptional activation domain constructs.