Fig. 4. Identification of lens proteins that associate with the γEt-crystallin and γBnop-crystallin nuclear deposits. (A) E17.5 Crygenop lenses were stained with polyclonal rabbit antibodies to CP49 (green channel) and counterstained with propidium iodide to detect chromatin (red channel). The CP49 localizes to the intranuclear inclusions, suggesting that the nuclei have activated those apoptotic-like responses found in normal lens fibre cell differentiation. (B and C) Cryosections from E15.5 wild-type (B) and Cryget (C) mice were stained with polyclonal antibodies to Prox1 (green channel) and counterstained with propidium iodide (red channel). The nuclei of the wild-type lens, including the central fibre cells (B, arrow), are positive for Prox1, whilst in the Cryget lens, these same nuclei are clearly Prox1 negative (C, arrow). Prox1 expression is lost from all of the fibre cells in the Cryget lens, except for the most recently differentiated cells closest to the lens bow (arrowheads).