Fig. 8. CRM1 contributes to nuclear exclusion of several translation factors. Stably transduced BHK cells expressing the indicated translation factors were analysed. The translation factors were tagged with either GFP alone or with GFP–NLS (to increase the nuclear influx rate). The distribution of the fusion proteins was analysed by laser scanning microscopy without or after LMB treatment (5 ng/ml for 30 min).