Fig. 1. Binding of proteins from HeLa cell extract to Exp5. HeLa cell extract (lane 1) was incubated with immobilized 2zExp5 (lanes 2 and 3) or 2zExp-t (lanes 4 and 5) in the absence or presence of RanQ69L(GTP). Bound proteins were separated by SDS–PAGE and detected by Coomassie Blue staining. The arrow indicates the 50 kDa protein that was excised, subjected to mass spectrometry and identified as eEF1A.