Table 2.

Leading-edge proteins contributing to the strongest negatively and positively enriched mitochondrial pathways in old male HFpEF cardiomyocyte mitochondria. Pre-ranked GSEA of MitoCarta 3.0 pathways was performed comparing old male HFD+L-NAME-treated mice with age-matched RD controls. The four pathways with the most negative normalized enrichment scores (NES) and the three pathways with the most positive NES were selected for presentation. The leading-edge protein subset that contributes most to the enrichment score for each pathway is shown. Negative NES indicates relative suppression in HFpEF, whereas positive NES indicates relative enrichment in HFpEF. FDR-adjusted P values are provided for each pathway.

Carbohydrate metabolism	TCA cycle	Amino acid metabolism	Complex I subunits	Xenobiotic metabolism	Mitochondrial ribosome assembly	Apoptosis
NES = −1.838	NES = −1.794	NES = −1.663	NES = −1.649	NES = 0.960	NES = 0.879	NES = 0.651
FDR = 0.013	FDR = 0.013	FDR = 0.013	FDR = 0.013	FDR = 0.090	FDR = 0.158	FDR = 0.231
Pdk4	Sdhd	Aldh18a1	Ndufv3	Ephx2	Eral1	Aifm2
Hmgcs2	Suclg2	Sfxn3	mt-Nd3	Mgst1	Ddx28	Bnip3
Pck2	Sdhc	Comt	Ndufa2	Maoa	Dhx30	Diablo
Sfxn5	Idh3a	Maoa	Ndufs6	Cyb5r3	Mtg1	Bcl2l13
Sdhd	Idh3g	Hibadh	Ndufb2	Acsm5	Mrm3	Htra2
Abhd11		Ldhb	Ndufb5	Mtarc2	Mrm2	Bcl2
Pdp2		Slc25a15	Ndufa7	Mpst	Mettl17	Cycs
Slc25a1		Slc25a29	Ndufa3	Bphl	Fastkd2	Endog
Suclg2		Adhfe1	Ndufb3	Maob	Ptcd3	Bak1
Fahd1		Aldh9a1	Ndufab1	Gstk1	Nsun4	Chchd2
Hadhb		Maob	Ndufa5	Cyb5b	Grsf1	Ghitm
Sdhc		Ppm1k		Tst		Aifm1
Mpc2		Hadhb		Abhd10
Pdk2		Etfdh
Ldhd		Grhpr
Me3		Hadha
Idh3a		Gls
Pdk1		Hibch
Acaa2		Shmt2
Acat1		Acaa2
Idh3g		Slc25a44
D2hgdh		Bcat2
Bdh1		Acat1
Mpc1		Prodh
Clybl		Hadh
Mmut		Bckdk
Auh		Ivd
Mdh2		Etfb